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Table 1 Serum and CSF Electrophoresis and GAD65 Antibody Titers

From: A case of stiff dog syndrome associated with anti-glutamic acid decarboxylase antibodies

Sample

Total Protein (g/dl)

Albumin (%)

Globulins (%)

Anti-GAD65 Antibody Titer (IU/ml)

α - 1

α - 2

β

γ

Negative Control Canine Seruma

6.9 ± 1.0

44.7 ± 3.9

11.3 ± 2.4

14.9 ± 3.1

21.6 ± 3.5

8.5 ± 1.0

8.0 ± 1.2

SDS Canine Serum Day 1

8.1

37.1

8.2

12.2

17.8

24.7

348 ± 25.1

SDS Canine Serum Day 6

7.4

41.2

8.4

11.6

19.5

19.3

77 ± 9.5

SDS Canine Serum Day 25

7.5

NA

NA

NA

NA

NA

106 ± 17.1

SDS Canine Serum Day 100

7.8

NA

NA

NA

NA

NA

46 ± 13.1

Human AntiGAD-65 Ab Positive Serum

7.2

NA

NA

NA

NA

NA

38 ± 8.5

Control CSFa

0.018 ± 2.4

39.7 ± 3.5

11.3 ± 1.5

15.8 ± 1.7

20.6 ± 3.3

7.6 ± 1.2

ND

SDS Canine CSF Day 1

0.1

NA

NA

NA

NA

NA

NA

SDS Patient CSF Day 6

0.025

39.2

8.5

14.6

18.2

19.5

54 ± 8.0

  1. IDDM Insulin-dependent diabetes mellitus, A not assayed, ND not detectable; all replicates below detection threshold and not different than assay blanks
  2. aControl values represent means +/− SD obtained from samples obtained from 6 healthy female beagle dogs < 3 years of age